Generate finished genome assemblies with the PacBio® RS
Faster and more cost-effective DNA sequencing technologies are accelerating investigations of a broad range of genomes – from model organisms to pathogens that may have a direct impact on human health. However, only a limited number of species have been completely sequenced due to the inherent limitations of second-generation sequencing technologies, including GC-bias and the inability to resolve large structural variations. A complete genome is needed to fully understand the biological relevance of an organism.
The complexity of genome assembly is driven by a variety of factors, including the length and number of total fragments and the presence of repeat regions. The PacBio RS single-molecule, real-time sequencing system provides extra-long read lengths that simplify and improve genome assembly. By reducing the number of contigs and producing superior consensus accuracy, researchers can finish genomes at a lower cost and with higher quality data.
PacBio RS benefits:
- Complete genome assemblies – long read lengths combine with high accuracy to produce high quality, finished genomes
- Accurate characterization of large structural variations – long read lengths uniquely provide the ability to sequence large repeat regions and resolve complex structure
- Unbiased genome coverage – balanced coverage and minimal GC-bias for high-quality assembly of high or low GC organisms or regions
- Cost-effective and fast – 10x reduction in finishing costs, results in less than a day, and no need for DNA amplification
