Recent advancements in our understanding of biological complexity have propelled the development of new tools. In the field of DNA sequencing, next-generation sequencers have dramatically increased productivity and provided novel insights into the structure and function of the genome. Despite these advances, researchers continue to face challenges in finishing genomes, characterizing variations, and understanding the function of key biological markers that may affect human health.
The PacBio RS II sequencing technology resolves single molecules in real time, allowing observation of structural and cell type variation not accessible with other technologies. These unique capabilities of the PacBio RS II system are ideally suited for a variety of applications, from De Novo assembly and targeted sequencing to detecting base modifications.
This novel approach to DNA sequencing can improve scientists' ability to understand health and disease processes, provide new mechanisms to diagnose and treat medical conditions, and enhance efforts to improve the world's food and energy supply. As more scientists adopt the SMRT technology, we expect numerous other applications of sequencing to become feasible for the first time.