Reagents

PacBio RS II reagent kits are available in several packaging options emphasizing the granularity and flexibility of our system. You can sequence DNA samples in an individual SMRT Cell or across multiple SMRT Cells according to your experiment design and data needs. This customizable run capability provides for cost efficiencies in both large and small sequencing projects.

The PacBio RS II front-end preparation and on-instrument sequencing workflow is supported by three reagent kits.

Front End Preparation
The DNA template preparation and DNA polymerase binding steps may be performed manually at the benchtop or by an automated liquid-handling system.

    1. SMRTbell Template Preparation Reagent Kits
        DNA and cDNA samples are converted into primer-annealed SMRTbell library format.

    2. DNA Polymerase Binding Reagent Kits
        Prepared DNA samples, in SMRTbell library format, are bound to the DNA polymerase         in preparation for sequencing.

DNA Sequencing
The DNA sequencing step is performed on the PacBio RS II. Sequencing reagent kits supply all materials needed for easy instrument loading.

    3. DNA Sequencing Reagent Kit
        Supports automated, on-instrument DNA sequencing. Used in conjunction with SMRT         Cell 8Pacs.

Sample prep kits

The SMRTbell Template Prep Kit 1.0 contains reagent supplies to perform SMRTbell library preparations of primer-annealed SMRTbell libraries for insert sizes ranging from 500 bp to over 20 kb. Reagent quantities support 10 library preparations.

The SMRTbell HT Template Prep Kit 1.0 contains reagent supplies to perform 96 automated SMRTbell library preparations of primer-annealed SMRTbell libraries for insert sizes ranging from 500 bp to over 20 kb. Reagents come in 4 x 24 reaction sets.
The reagents are compatible with protocols that have been co-developed for the Agilent® NGS Workstation Option B and the Sciclone® NGSx Workstation from PerkinElmer. The available protocols for these systems have been validated with the PacBio 10 kb Template preparation workflow.
To learn more, please contact your local Field Application Scientist (FAS) or Sales Representative.

AMPure PB magnetic beads are specifically formulated for the SMRT sequencing workflow. Beads are used for DNA cleanup and purification during sample extraction and at various stages in the workflow.



Binding kits

The DNA/Polymerase Binding Kit P6 contains reagent supplies to bind prepared DNA template libraries to the DNA polymerase P6 in preparation for sequencing on the PacBio RS. Reagent quantities support 24 binding reactions. DNA Polymerase P6 should be used only in conjunction with Sequencing Reagent 4.0.

The DNA/Polymerase Binding Kit P5 contains reagent supplies to bind prepared DNA template libraries to the DNA polymerase P5 in preparation for sequencing on the PacBio RS II. Reagent quantities support 24 binding reactions. DNA Polymerase P5 should be used only in conjunction with Sequencing Reagent 3.0.

The DNA/Polymerase Binding Kit P4 contains reagent supplies to bind prepared DNA template libraries to the DNA polymerase P4 in preparation for sequencing on the PacBio System. Reagent quantities support 24 binding reactions. DNA Polymerase P4 should be used only in conjunction with Sequencing Reagent 2.0.

The MagBead Kit contains reagent supplies to capture DNA polymerase and template complex for loading onto the PacBio RS. Kit components include: MagBeads and MagBead Buffer kit.



Sequencing reagents and kits

The DNA Sequencing Kit 4.0 contains a sequencing reagent plate with C3 chemistry and OS Enzyme for Single-Molecule Real-Time sequencing on the PacBio RS. Reagent quantities support 8 sequencing reactions to be used in conjunction with SMRT Cell 8Pac(s) and DNA Polymerase P6.

The DNA Sequencing Bundle 4.0 contains reagent and disposable supplies for 80 reactions of Single-Molecule Real-Time sequencing. It contains: 10 DNA Sequencing Kits 2.0, 1 SMRT Cell Oil pack, 1 Sequencing Plate Septa pack, 1 Tube Septa pack.

The DNA Sequencing Kit 3.0 contains a sequencing reagent plate with C3 chemistry and OS Enzyme for single molecule real‐time sequencing on the PacBio RS II. Reagent quantities support 8 sequencing reactions to be used in conjunction with SMRT® Cell 8Pac(s) and DNA Polymerase P5.

The DNA Sequencing Bundle 3.0 contains reagent and disposable supplies for 80 reactions of single molecule real‐time sequencing. It contains: 10 DNA Sequencing Reagents, 1 SMRT Cell Oil pack, 1 Sequencing Plate Septa pack, 1 Tube Septa pack.

The DNA Sequencing Reagent contains a sequencing reagent plate with C2 chemistry and OS Enzyme for single molecule real‐time sequencing on the PacBio System. Reagent quantities support 8 sequencing reactions to be used in conjunction with SMRT® Cell 8Pac(s) and DNA Polymerase P4.

The DNA Sequencing Bundle 2.0 contains reagent and disposable supplies for 80 reactions of single molecule real‐time sequencing. It contains: 10 DNA Sequencing Reagents, 1 SMRT Cell Oil pack, 1 Sequencing Plate Septa pack, 1 Tube Septa pack.

SMRT® Cell Oil contains 10 reagent tubes for single molecule, real‐time DNA sequencing on the PacBio® System to be used in conjunction with the DNA Sequencing Kit. Reagent quantities support 10 sequencing runs.



Sequencing Controls

The DNA Internal Control Complex contains a fixed template of 2 kb length, bound to DNA Polymerase P6 for use as an internal sequencing control. Reagent quantities provide spike-in controls for a minimum of 24 samples.

The Lambda Lib Complex (2Kb) contains Lambda library DNA template sheared to a peak size 2Kb bound to a DNA polymerase.