Kinnex

What is Kinnex?

The Kinnex kits are based on the MAS-Seq method, which concatenates smaller amplicons into larger fragment libraries for throughput increase.

Kinnex kits increase throughput for full-length RNA, single-cell RNA, and 16S rRNA sequencing on PacBio long-read sequencing systems to enable large-scale studies at a resolution difficult to attain with short-read RNA sequencing.

Want to watch more Kinnex webinars? Learn from tutorials? See how it’s being used by other researchers? Check out the Kinnex YouTube playlist.

View brochure Watch Kinnex playlist

Long-read value for RNA applications

Application	Full-length RNA	Single-cell RNA	16S rRNA
Sequencing goal	Isoform discovery and abundance information	Cell type-specific isoform isoform discovery and abundance information	Species identification in metagenomics samples
Short-read shortcomings	Incorrect isoform discovery and abundance information	No isoforms. Only genes	No species identification
Other long-read drawbacks	Lower accuracy poor transcript identification and variant calling	Lower accuracy poor transcript identification and variant calling	Lower accuracy poor transcript identification and variant calling
HiFi advantages	Isoform discovery and abundance information	Cell type-specific isoform discovery	Species-level identification
	Read the competitive whitepaper	Read the competitive whitepaper	Read the app note

“The new family of Kinnex kits for RNA sequencing are designed to provide customers with cost-effective, end-to-end kitted solutions to support their research.”
Christian Henry, President and CEO of PacBio

Kinnex application kits


	Kinnex full-length RNA kit	Kinnex single-cell RNA kit	Kinnex 16S rRNA kit
Kit advantage	Full-length isoform discovery with flexible sample multiplexing	Cell type-specific isoform isoform discovery with the highest accuracy	Full-length 16S rRNA for species identification
Total throughput	15-20M (Sequel II) 30-40M (Revio)	30-40M (Sequel II) 80-100M (Revio)	20-25M (Sequel II) 50-60M (Revio)
Sample multiplexing recommendation*	5M × 3-plex (Sequel II) 10M × 4-plex (Revio)	1-plex (Sequel II) 1- or 2-plex (Revio)	384-plex (Sequel II) 384- to 1,536-plex (Revio)
Analysis	SMRT Link v13.0 and above	To be released	SMRT Link v13.0 and above
	Read the app note Read the tertiary tools app note	Read the app note Read the tertiary analysis wiki	Read the app note Try the 16S analysis workflow
* Sample multiplexing and read depth recommendation will vary by experimental goal, tissue, and species.

On-demand webinar

Leveraging isoform-level RNA sequencing to understand rare disease pathogenesis

In this webinar, Dr. Carolina Jaramillo Oquendo, a Research Fellow at the University of Southampton, presents her analyses of rare disease samples, in collaboration with the University Hospital Southampton NHS Foundation Trust. Dr. Oquendo also discusses her groundbreaking work demonstrating how transcriptomics, utilizing the new full-length Kinnex RNA kit, has been instrumental in revealing the underlying biology in samples from individuals with rare diseases where short-read genome sequencing did not provide an explanation.

Watch webinar

RNA sequencing datasets

Application	Dataset	Download literature	Technology	Sequencing system
Kinnex single-cell	Homo sapiens - PBMC 10x Chromium Single Cell (5' and 3' libraries)	Kinnex single-cell application note	HiFi long-read	Sequel II and Revio systems
Kinnex single-cell	Homo sapiens - HG002 (10x 5')	Kinnex single-cell RNA application note	HiFi long-read	Revio system
Kinnex full-length RNA	Homo sapiens – UHRR	Kinnex full-length RNA application note	HiFi long-read	Sequel II and Revio systems
Kinnex full-length RNA	Homo sapiens – HG002	Kinnex full-length RNA application note	HiFi long-read	Revio system
Kinnex full-length RNA	Homo sapiens - Heart	Kinnex full-length RNA application note	HiFi long-read	Revio system
Kinnex full-length RNA	Homo sapiens - Cerebellum	Kinnex full-length RNA application note	HiFi long-read	Revio system